Zinc tetraaminophthalocyanine-Fe3O4 nanoparticle composite for laccase immobilization.

摘要: Zinc tetraaminophthalocyanine-Fe3O4 nanoparticle composites were prepared by organic-inorganic complex technology and characterized. It has been proved that the ZnTAPc dispersed randomly onto surface of Fe3O4 nanoparticles to form molecular dispersion layer there was a relatively strong bond between central zinc cation oxygen. The composite took shape roundish spheres with mean diameter about 15 nm. Active amino groups magnetic carriers could be used bind laccase via glutaraldehyde. optimal pH for activity immobilized laccases free same at 3.0 temperature immobilization on ZnTAPc-Fe3O4 45°. yields Km value 25% 20.1 μM, respectively. This kind good thermal, storage operation stability, as sensing biocomponent fiber optic biosensor based enzyme catalysis.