Efficient recombinant production of prodigiosin in Pseudomonas putida

摘要: Serratia marcescens and several other bacteria produce the red-colored pigment prodigiosin which possesses bioactivities as an antimicrobial, anticancer immunosuppressive agent. Therefore, there is a great interest to this natural compound. Efforts aiming at its biotechnological production have so far largely focused on original producer opportunistic human pathogen S. marcescens. Here, we demonstrate efficient in heterologous host Pseudomonas putida. Random chromosomal integration of 21 kb biosynthesis gene cluster P. putida KT2440 was employed construct constitutive strains. Standard cultivation parameters were optimized such that titers 94 mg/L culture obtained upon growth 20 °C using rich medium under high aeration conditions. Subsequently, novel, fast effective protocol for extraction purification established enabling straightforward isolation from medium. In summary, describe here highly method biosynthetic may serve basis large amounts bioactive compound provide platform further in-depth studies prodiginine biosynthesis.