A novel in vitro pancreatic carcinogenesis model

摘要: Abstract Environmental factors (e.g., BaP) have been pointed out as one of the etiologies pancreatic cancer. However, very limited experimental assays are available to identify specific environmental mutagens or susceptibility genes. In this study, we developed a simple in vitro cell culture model system that can be used study molecular and biochemical aspects carcinogenesis near-normal immortalized ductal epithelial lines. order demonstrate xenobiotic stress response is intact these cells, employed standard biology techniques. For examples, luciferase reporter and/or real-time quantitative PCR were determine stress-induced CYP1A1 CYP1B1 gene expression. Western blotting immunocytochemistry TCDD BaP could activate AhR signaling. exploring mechanism, incubated cells with [ 3 H]BaP determined BaP–DNA binding activity by measuring its radioactivity. adduct formation was further confirmed 32 P]-postlabeling assay. Finally, demonstrated effects endogenous BRCA1 accumulation our system. As results, no apparent assay found either control-siRNA AhR-siRNA pretreated cells. On other hand, significant increase knockdown conclusion, suggest may provide feasibility for future studies on basis caused dietary mutagens.