Aflatoxin B1 promotes autophagy associated with oxidative stress-related PI3K/AKT/mTOR signaling pathway in mice testis
作者： Wanyue Huang , Zheng Cao , Jian Zhang , Qiang Ji , Yanfei Li
DOI: 10.1016/J.ENVPOL.2019.113317
关键词:
摘要: Abstract Aflatoxin B1 (AFB1) is a hazard environmental pollutants and the most toxic one of all aflatoxins. AFB1 can cause serious impairment to testicular development spermatogenesis, yet underlying mechanisms remain inconclusive. Oxidative stress acts as master mechanism toxicity, promote autophagy. Abnormal autophagy resulted in damage spermatogenesis disorders. The objective this study was explore effect on mice testis its potential mechanisms. In study, male were intragastrically administered with 0, 0.375, 0.75 or 1.5 mg/kg body weight for 30 days. We found that induced damage, reduced serum testosterone level impaired sperm quality accompanied elevation oxidative germ cell apoptosis. Interestingly, we observed increasing numbers autophagosomes AFB1-exposed testis. Meanwhile, caused abnormal characterization increased expressions LC3, Beclin-1, Atg5 p62. Furthermore, downregulated PI3K, p-AKT p-mTOR Taken together, our data indicated promoted autophagy, which associated stress-related PI3K/AKT/mTOR signaling pathway
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Heather Strosnider, Eduardo Azziz-Baumgartner, Marianne Banziger, Ramesh V Bhat, Robert Breiman, Marie-Noel Brune, Kevin DeCock, Abby Dilley, John Groopman, Kerstin Hell, Sara H Henry, Daniel Jeffers, Curtis Jolly, Pauline Jolly, Gilbert N Kibata, Lauren Lewis, Xiumei Liu, George Luber, Leslie McCoy, Patience Mensah, Marina Miraglia, Ambrose Misore, Henry Njapau, Choon-Nam Ong, Mary TK Onsongo, Samuel W Page, Douglas Park, Manish Patel, Timothy Phillips, Maya Pineiro, Jenny Pronczuk, Helen Schurz Rogers, Carol Rubin, Myrna Sabino, Arthur Schaafsma, Gordon Shephard, Joerg Stroka, Christopher Wild, Jonathan T Williams, David Wilson, None, Workgroup Report: Public Health Strategies for Reducing Aflatoxin Exposure in Developing Countries Environmental Health Perspectives. ,vol. 114, pp. 1898- 1903 ,(2006) , 10.1289/EHP.9302
J I Ogonar, N Uraih, I N Ibeh, Dietary exposure to aflatoxin in human male infertility in Benin City, Nigeria. International journal of fertility and menopausal studies. ,vol. 39, pp. 208- 214 ,(1994)
Noha Gamal Bahey, Hekmat Osman Abd Elaziz, Kamal Kamal El Sayed Gadalla, Toxic effect of aflatoxin B1 and the role of recovery on the rat cerebral cortex and hippocampus. Tissue & Cell. ,vol. 47, pp. 559- 566 ,(2015) , 10.1016/J.TICE.2015.09.001
S. H. Henry, PUBLIC HEALTH:Reducing Liver Cancer--Global Control of Aflatoxin Science. ,vol. 286, pp. 2453- 2454 ,(1999) , 10.1126/SCIENCE.286.5449.2453
Feifan Zhou, Ying Yang, Da Xing, Bcl-2 and Bcl-xL play important roles in the crosstalk between autophagy and apoptosis FEBS Journal. ,vol. 278, pp. 403- 413 ,(2011) , 10.1111/J.1742-4658.2010.07965.X
Päivi Ylä‐Anttila, Helena Vihinen, Eija Jokitalo, Eeva‐Liisa Eskelinen, Monitoring autophagy by electron microscopy in Mammalian cells. Methods in Enzymology. ,vol. 452, pp. 143- 164 ,(2009) , 10.1016/S0076-6879(08)03610-0
Xiao Huan Liang, Saadiya Jackson, Matthew Seaman, Kristy Brown, Bettina Kempkes, Hanina Hibshoosh, Beth Levine, None, Induction of autophagy and inhibition of tumorigenesis by beclin 1 Nature. ,vol. 402, pp. 672- 676 ,(1999) , 10.1038/45257
Jonathan T. Busada, Bryan A. Niedenberger, Ellen K. Velte, Brett D. Keiper, Christopher B. Geyer, Mammalian target of rapamycin complex 1 (mTORC1) Is required for mouse spermatogonial differentiation in vivo Developmental Biology. ,vol. 407, pp. 90- 102 ,(2015) , 10.1016/J.YDBIO.2015.08.004
Azza M. Mohamed, Nadia S. Metwall, Antiaflatoxigenic activities of some plant aqueous extracts against aflatoxin-B1 induced renal and cardiac damage. Journal of Pharmacology and Toxicology. ,vol. 4, pp. 1- 16 ,(2009) , 10.3923/JPT.2009.1.16
N MATHURIA, R VERMA, Curcumin ameliorates aflatoxin-induced toxicity in mice spermatozoa. Fertility and Sterility. ,vol. 90, pp. 775- 780 ,(2008) , 10.1016/J.FERTNSTERT.2007.07.1300