Influence of plant genotype and soil on the wheat rhizosphere microbiome: identification of a core microbiome across eight African and European soils

摘要: Abstract An avenue to improve cereal yields is harness and manipulate the plant microbiome increase crop nutrition resistance pathogens abiotic stressors. To develop engineering solutions, more research needed characterize microbiomes across contrasted geographical locations, agricultural practices genotypes. Previous work on wheat focused bacterial diversity marginally archaeal fungal diversity. Hence, no integrative assessment of rhizospheric including protist (i.e. amoeba, ciliates, stramenopiles) currently available. Here, we characterized rhizosphere by considering both prokaryotic (archaea bacteria) eukaryotic (fungi protists) communities in soils from four different countries (Cameroon, France, Italy, Senegal). The goals this study were determine influence genotype, (conventional vs organic) soil type microbiome. additional goal was if a core existed these countries. We found limited effect genotype (explain 2% variance) observed that majority microbial taxa consistently associated multiple genotypes grown same soil. Large differences richness structure between eight studied (57% two (10% variance). However, despite composition soils, 179 (2 archaea, 104 bacteria, 41 fungi, 32 roots, constituting In addition being prevalent, few highly abundant collectively represented 50% relative abundance our entire dataset. provide evidence propose “most wanted” list, 9 hubs (potential keystone taxa), should be targets for future culturomics, metagenomics creation synthetic microbiomes. Additionally, confirm protists are an integral part holobiont encourage their roles controlling diseases.