Capabilities and limitations of gel electrophoresis for elemental speciation: A laboratory's experience

摘要: Gel electrophoresis is a fractionation/separation technique that yields valuable information in the field of metalloproteomics, often referred to as metallomics. This paper based on four years practical experience authors' lab this domain and highlights capabilities limitations gel electrophoresis. Pitfalls were recognized by identifying origin artefacts separation, species degradation being most important. can be accomplished under either native or denaturing conditions. The speciation vanadium selenium among serum yeast proteins, respectively, sued illustrate these two major modes. powerful approach two-dimensional review methods used our laboratory also descries application detection techniques, autoradiography one hand electrothermal vaporization - laser ablation (LA)-inductively coupled plasma-mass spectrometry (ICP-MS) other.