Cell-to-cell variability in the yeast pheromone response: high throughput screen identifies genes with different effects on transmitted signal and response

摘要: Populations of isogenic cells often respond coherently to signals despite differences in protein abundance and cell state. Our previous work the Saccharomyces cerevisiae pheromone response system (PRS) uncovered processes that reduced cell-to-cell variation signal response. To understand these other controlled variation, we generated a whole-genome collection haploid strains with deletions non-essential genes used high-throughput flow cytometry screen more than 1000. We identified 50 'variation genes' required for normal variability Some affected only variability, strength, or output, defining quantities as separable 'axes' behavior. Two cytoplasmic microtubule function.