Basic Information Regarding the HPLC Techniques

摘要: Abstract Basic information about instrumentation in HPLC includes presentation of the solvent supply system, pumping injectors and autosamplers, analytical columns, detectors their selection a configuration, control data processing unit. Parameters describing chromatographic process, retention, quantitation are included this chapter. Quantitation influence sample volume analyte amount injected column on results detailed. Also, retention elution mechanisms different types separation presented, main concepts related to subject discussed, such as thermodynamic aspects partition adsorption equilibria, role polarity mechanisms, solvophobic theory. The pH, temperature additives equilibria discussed according literature focused these issues.