A microfluidic-based genetic screen to identify microbial virulence factors that inhibit dendritic cell migration
作者： Laura M. McLaughlin , Hui Xu , Sarah E. Carden , Samantha Fisher , Monique Reyes
DOI: 10.1039/C3IB40177D
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摘要: Microbial pathogens are able to modulate host cells and evade the immune system by multiple mechanisms. For example, Salmonella injects effector proteins into evades in part inhibiting dendritic cell (DC) migration. The identification of microbial factors that normal functions should lead development new classes therapeutics target these pathways. Current screening methods identify either or pathogen genes involved modulating migration towards a chemical signal limited because they do not employ stable, precisely controlled gradients. Here, we develop positive selection microfluidic-based genetic screen allows us virulence manipulate DC within linear chemokine Our identified 7 effectors (SseF, SifA, SspH2, SlrP, PipB2, SpiC SseI) inhibit chemotaxis toward CCL19. This method is widely applicable for identifying novel influence as well revealing mammalian directed
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