Permeability and biocompatibility of a new hydrogel used for encapsulation of hepatocytes

摘要: A new high-water-content (83%) and highly permeable anionic polyelectrolyte hydrogel was obtained by phase inversion of a polymer solution containing 6% polyacrylonitrile-sodium methallylsulphonate, 91% dimethylsulphoxide 3% physiological saline solution. Hydrogel-based hollow fibres (HFs) were fabricated with co-extrusion apparatus in collaboration Hospal (France). HFs have an internal diameter 800μm wall thickness 100μm. Experimental results demonstrated that hydrogel-based to albumin (mol. wt 69000) human immunoglobulin G (150000), but impermeable immunoglobulins (170000) M (900000) after 24 h diffusion. In vitro, the viability isolated rat hepatocytes injected into 64 ± 10 d versus 30 5% for cultured Petri dishes (P = 0.0001). Under these conditions, amount released encapsulated 12 3 μ/24 h/106 cells at day 10, whereas time no dishes. vivo, histological study implanted up 6wk peritoneum rats revealed low inflammatory tissue reaction without giant multinucleate foreign tissue, which decreased third week. The survival rate over 85% 45d transplantation syngeneic Lewis rats. Therefore, this demonstrates favourable properties encapsulation regard its biocompatibility, permeability ability maintain functional state prolonged periods.