Immunological response after WT1 mRNA-loaded dendritic cell immunotherapy in ovarian carcinoma and carcinosarcoma.
作者： Stefaan W Van Gool , An Coosemans , Anke Vanderstraeten , Ignace Vergote , Philippe Moerman
DOI:
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摘要: Background Dendritic cell (DC)-based immunotherapy is an emerging new treatment option in ovarian cancer, important cause of cancer-related mortality. Patients and methods One patient with carcinosarcoma (OCS) one serous cancer (SOC) received four weekly vaccinations autologous DCs electroporated mRNA coding for the Wilms' tumor gene 1 (WT1). Safety, feasibility immunogenicity were assessed. Results Vaccination was feasible without toxicity. In ex vivo antigen re-stimulation assay peripheral blood mononuclear cells, both patients showed increasing cluster differentiation 137 (CD137+) antigen-specific T-cells interleukin 10 (IL-10) production post-vaccination. Moreover, interleukin-2 (IL-2) increased as well interferon-gamma (IFN-γ) necrosis factor-alpha (TNF-α) (SOC). Disease progressed after vaccines continued conventional therapies. After cessation immunotherapy, they had extended survival 19 12 months. Conclusion To our knowledge, we report first time T-cell WT1 mRNA-loaded DC cancer.
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