Electrophoretic characterization and purification of silica-coated photon-upconverting nanoparticles and their bioconjugates.

摘要: Photon-upconverting nanoparticles (UCNPs) have attracted much interest as a new class of luminescent label for the background-free detection in bioanalytical applications. UCNPs and other are commonly coated with silica shell to improve their dispersibility chemical stability aqueous buffer incorporate functional groups subsequent bioconjugation steps. The process coating, however, is difficult control without suitable analytical preparative methods. Here, we introduced agarose gel electrophoresis analysis purification silica-coated UCNPs. can be doped fluorescent dye direct influencing structure or electrophoretic mobility nanoparticles. preparation bare by reverse microemulsion resulted individual but also distinct aggregates that could separated isolated from gel. In contrast, an ultrathin ...