Lens formation from cornea implanted into amputated hindlimbs of Xenopus laevis larvae requires innervation or proliferating cell populations in the stump.

摘要: The capacity of amputated early and late limbs larval Xenopus laevis to promote lens-forming transformations corneal implants in the absence a limb regeneration blastema has been tested by implanting outer cornea fragments from donor larvae at stage 48 (according Nieuwkoop Faber 1956), into stumps 52 57. Blastema formation prevented either covering amputation surface with skin or reconnecting part stump. Results show that non-regenerating could lens not only when innervated but also denervated. A similar result was observed 57 where In this case, relevant tissue dedifferentiation boundary region between stump autografted limb. However, limbs, skin, innervated. no tissues observed. These results indicate is prerequisite for implanted hindlimbs X. can be promoted factors delivered nerve fibres produced populations undifferentiated dedifferentiated cells.