Epidermal Growth Factor Receptor Potentiates MCM7-Mediated DNA Replication through Tyrosine Phosphorylation of Lyn Kinase in Human Cancers

摘要: Epidermal growth factor receptor (EGFR) initiates a signaling cascade that leads to DNA synthesis and cell proliferation, but its role in regulating replication licensing is unclear. Here, we show activated EGFR phosphorylates the p56 isoform of Lyn, p56(Lyn), at Y32, which then MCM7, critical for replication, Y600 increase association with other minichromosome maintenance complex proteins, thereby promoting assembly proliferation. Both p56(Lyn) Y32 MCM7 phosphorylation are enhanced proliferating cells correlated poor survival breast cancer patients. These results establish enhances through Lyn human cells.