Investigation of Tropane Alkaloids in Genetically Transformed Atropa belladonna L. Cultures

摘要: The purpose of the present study was to determine tropane alkaloid content genetically transformed hairy root cultures Atropa belladonna L. Determination alkaloids performed by HPLC method. Samples were extracted with chloroform – methanol - cc. ammonia 15:5:1 (v/v/v). Crude extracts purified on Extrelut columns. separation Luna C8 reversed phase column. An isocratic mixture acetonitrile 30 mM phosphate buffer 12.2:79.7:8.1(v/v/v) used as eluent. Peaks identified addition standards and diode-array detection. Hyoscyamine, scopolamine apoatropine determined external standard method at 210 nm. We measured in vitro (hairy roots reorganised plants) cultivated Gamborg B5 basic media. highest hyoscyamine found clone #K5 (0,223 m/m%) #K4 (0,018 respectively. Alkaloid contents higher than plants.