Specific contacts between the FLP protein of the yeast 2-micron plasmid and its recombination site.
作者： R C Bruckner , M M Cox
DOI: 10.1016/S0021-9258(18)67314-4
关键词:
摘要: Contact points between the FLP protein of yeast 2-micron plasmid and its recombination site have been defined. Important features region previously defined as minimal in vitro include a pair 13-base inverted repeats separated by an 8-base spacer. The two protein-binding sites within this are 12 base pairs length. In each case they internal 11 one repeats, well adjacent 6 spacer not involved binding or recognition protein. When size is increased decreased pair, distance cleavage also correspondingly pair. Points unaffected changes sequence. Specific contact involving purine residues, identified methylation protection interference experiments, located both major minor grooves DNA. Additional phosphate groups phosphate-deoxyribose backbone clustered near sites.
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