Characterization of a novel broad-spectrum antifungal protein from virus-infected Helminthosporium (Cochliobolus) victoriae.
作者： Patricia B. de Sá , Wendy M. Havens , Said A. Ghabrial
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摘要: ABSTRACT A broad-spectrum anti-fungal protein of ≈10 kDa, designated victoriocin, was purified from culture filtrates a virus-infected isolate the plant-pathogenic fungus Helminthosporium victoriae (teleomorph: Cochliobolus victoriae) by multistep procedure involving ultrafiltration and reverse-phase high-performance liquid chromatography (RP-HPLC). Amino acid sequences, obtained automated Edman degradation sequencing RP-HPLC-purified victoriocin-derived peptides, were used to design primers for degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR) amplification H. DNA cDNA templates. An open reading frame coding victoriocin precursor 183 amino acids with calculated molecular mass ≈20 kDa amplified PCR genomic but not control Penicillium chrysogenum. Southern hybridization analysis confirmed presence gene in all strains tested. Sequence indicated that victo...
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