In situ PCR for detection and differentiation of Newcastle disease virus strains in Leghorn chickens

摘要: In experiment 1, six specific pathogen free (SPF) chickens were intra-nasally infected with velogenic (v) NDV strain titre of 10⁵ EID50/0.1 mL and 6 non-infected birds used as controls. Chickens sacrificed at different times tissue samples collected for situ PCR immunoperoxidase staining (IPS). was more sensitive (P < 0.05) than IPS detection NDV. a 2nd experiment, done to differentiate strains. Groups 5 SPF each, (10⁵EID50/0.1 mL) or lentogenic (l) (103.0 Non-infected After sampling tissues, an developed using probes. probe positive only tissues by whereas infected-tissues. The findings suggested that the differentiated from virus