Photoregulated gene expression may involve ubiquitous DNA binding proteins.

摘要: Several promoter elements have previously been shown to influence the expression of cab-E gene in Nicotiana plumbaginifolia. Here we demonstrate, by electrophoretic mobility shift and methylation interference assays, that a complex pattern protein-DNA interactions characterizes this promoter. Among multiple proteins identified, focused on five different factors which either occupied important regulatory and/or were present relatively large amounts nuclear extracts. All these distinguished basis their recognition sequence other biochemical parameters. One, GBF, interacted with single within homologous G-box found many photoregulated plant promoters. A second factor, GA-1, bound GATA element is located between CAAT TATA boxes all LHCII Type I CAB GA-1 also vitro I-boxes Arabidopsis rbcS-1A as-2 site CaMV 35S Two factors, GC-1 AT-1, sites localized GC-rich AT-rich elements, respectively. GT-1, protein interacts promoters light-regulated genes, seven distinct distributed throughout