Antibiotic Resistance Determinants in a Pseudomonas putida Strain Isolated from a Hospital

摘要: Environmental microbes harbor an enormous pool of antibiotic and biocide resistance genes that can impact the profiles animal human pathogens via horizontal gene transfer. Pseudomonas putida strains are ubiquitous in soil water but have been seldom isolated from humans. We established a collection P. in-patients different hospitals France. One (HB3267) kills insects is resistant to majority antibiotics used laboratories hospitals, including aminoglycosides, s-lactams, cationic peptides, chromoprotein enediyne antibiotics, dihydrofolate reductase inhibitors, fluoroquinolones quinolones, glycopeptide macrolides, polyketides sulfonamides. Similar other clinical isolates strain was sensitive amikacin. To shed light on broad pattern resistance, which rarely found this species, genome sequenced analysed. The study revealed determinants multiple both chromosomally-borne as well located pPC9 plasmid. Further analysis indicated has recruited environmental microorganisms opportunistic true pathogens. plasmid not self-transmissible, be mobilized by bacterial plasmids making it capable spreading new hosts.