Regeneration of biomimetic hydroxyapatite on etched human enamel by anionic PAMAM template in vitro
作者： Liang Chen , Kunneng Liang , Jianshu Li , Duo Wu , Xuedong Zhou
DOI: 10.1016/J.ARCHORALBIO.2013.03.008
关键词:
摘要: Abstract Objective To repair the demineralized enamel by biomimetic method, and effect of Poly (amido amine) (PAMAM) dendrimers on crystallization hydroxyapatite etched surface is investigated. Design PAMAM were synthesized step following classical method modified with carboxylic acid groups (COOH) surface. Demineralized human samples immersed in 10,000 ppm PAMAM-COOH solution for 30 min then calcium phosphorous or without fluoride under near-clinical conditions 20 h. Other as control group. After immersion, micro structure, morphology composition regrown crystals longitudinal transversal surfaces investigated SEM, XRD FTIR, results compared intact enamel. Results With templates, well-arranged rod-like formed they parallel to long axis crystals, which was more obvious Otherwise, irregular flake-like obtained PAMAM-COOH. Fluorapatite not influenced but its specific distribution also shown patterns temples spectra showed that main phase their structure close Amide I band two bands methylene detected FTIR demonstrated presence within coating. Conclusions It concluded can play organic template induce formation HAP same orientation mineral relatively short time.
sci-hub.se 参考文章(30)
G. K. Hunter, H. A. Curtis, M. D. Grynpas, J. P. Simmer, A. G. Fincham, Effects of recombinant amelogenin on hydroxyapatite formation in vitro. Calcified Tissue International. ,vol. 65, pp. 226- 231 ,(1999) , 10.1007/S002239900688
Fan Zhang, Zhuo-Hua Zhou, Shi-Ping Yang, Li-Hong Mao, Hong-Mei Chen, Xi-Bin Yu, Hydrothermal synthesis of hydroxyapatite nanorods in the presence of anionic starburst dendrimer Materials Letters. ,vol. 59, pp. 1422- 1425 ,(2005) , 10.1016/J.MATLET.2004.11.058
Liam C. Palmer, Christina J. Newcomb, Stuart R. Kaltz, Erik D. Spoerke, Samuel I. Stupp, Biomimetic Systems for Hydroxyapatite Mineralization Inspired By Bone and Enamel Chemical Reviews. ,vol. 108, pp. 4754- 4783 ,(2008) , 10.1021/CR8004422
M. Lijima, Y. Moriwaki, H.B. Wen, A.G. Fincham, J. Moradian-Oldak, Elongated Growth of Octacalcium Phosphate Crystals in Recombinant Amelogenin Gels under Controlled Ionic Flow Journal of Dental Research. ,vol. 81, pp. 69- 73 ,(2002) , 10.1177/002203450208100115
C. Robinson, S.J. Brookes, J. Kirkham, W.A. Bonass, R.C. Shore, Crystal growth in dental enamel: the role of amelogenins and albumin. Advances in Dental Research. ,vol. 10, pp. 173- 180 ,(1996) , 10.1177/08959374960100020901
Yuwei Fan, James R. Nelson, Jason R. Alvarez, Joseph Hagan, Allison Berrier, Xiaoming Xu, Amelogenin-assisted ex vivo remineralization of human enamel: Effects of supersaturation degree and fluoride concentration. Acta Biomaterialia. ,vol. 7, pp. 2293- 2302 ,(2011) , 10.1016/J.ACTBIO.2011.01.028
Mayumi Iijima, Chang Du, Christopher Abbott, Yutaka Doi, Janet Moradian-Oldak, Control of apatite crystal growth by the co-operative effect of a recombinant porcine amelogenin and fluoride. European Journal of Oral Sciences. ,vol. 114, pp. 304- 307 ,(2006) , 10.1111/J.1600-0722.2006.00324.X
Si-Jia Yan, Zhuo-Hua Zhou, Fan Zhang, Shi-Ping Yang, Lian-Zhun Yang, Xi-Bin Yu, Effect of anionic PAMAM with amido groups starburst dendrimers on the crystallization of Ca10(PO4)6(OH)2 by hydrothermal method Materials Chemistry and Physics. ,vol. 99, pp. 164- 169 ,(2006) , 10.1016/J.MATCHEMPHYS.2005.10.009
C. Robinson, S. Connell, S.J. Brookes, J. Kirkham, R.C. Shore, D.A.M. Smith, Surface chemistry of enamel apatite during maturation in relation to pH: implications for protein removal and crystal growth. Archives of Oral Biology. ,vol. 50, pp. 267- 270 ,(2005) , 10.1016/J.ARCHORALBIO.2004.11.017
Janet Moradian-Oldak, Chang Du, Giuseppe Falini, On the formation of amelogenin microribbons. European Journal of Oral Sciences. ,vol. 114, pp. 289- 296 ,(2006) , 10.1111/J.1600-0722.2006.00285.X