Human glomerular cells in culture. Three subcultured cell types bearing glomerular antigens.

摘要: In this paper, we describe three cell types from the explanted human glomerulus: circular glomerular (CGC), rhomboid (RGC), and small ovoid (SOGC). These cells were compared with subcultured umbilical vein endothelial smooth muscle cells, uterine skin fibroblasts. Immunochemical comparisons utilized antiserums to antigens in antiglomerular basement membrane, antifibroblast surface antigen (FSA) (reactive extensively mesangium), antiactomyosin (AMY) localizing more restrictively mesangium, antihemophilic factor (AHF) endothelium. No cultured bore AHF marker of cells. The epithelioid CGC excrete most GBM as an orderly palisade granules surface. FSA is rapidly lost RGC have a typical multilayered morphology prominent complex AMY pattern periodic aggregates fibrils. adheres SOGC form initial nonoverlapped monolayer resembling but elongate multilayers after confluency. fibril distinctively multidirectional. translation vitro characteristics into vivo identification must be interpreted cautiously: may derive epithelial cells; mesangial represent proliferating, less differentiated or another, unidentified, type.