Glypican-3 (GPC3) expression in human placenta: localization to the differentiated syncytiotrophoblast.

摘要: The expression of glypican-3 (GPC3), a heparan-sulfate proteoglycan associated with the Simpson-Golabi-Behmel fetal overgrowth syndrome, was studied in normal human placental tissue and cell lines derived from placentae. Cytotrophoblasts term placentae expressed GPC3 mRNA at low levels culture. increased markedly during trophoblast differentiation. By contrast, fibroblast did not express Similarly, choriocarcinoma (BeWo, JAR, JEG) failed to mRNA. In situ hybridization confirmed localization syncytiotrophoblast. Furthermore, immunohistochemical staining paraffin imbedded demonstrated intense syncytiotrophoblast layer less cytotrophoblasts. No mesenchymal elements noted. These data confirm presence placenta suggest it is by differentiated term.