An assay for the detection of the dna genome of hepatitis b virus in serum
作者： Mark Berninger , Marlene Hammer , Bill Hoyer , John L. Gerin
关键词:
摘要: An assay based on nucleic acid hybridization detects and quantitates hepatitis B virus (HBV) DNA in particles present serum. This allows rapid examination of multiple samples is sensitive reproducible; serum are treated with proteolytic enzyme detergent then extracted phenol chloroform. The deproteinized extracts which may contain HBV made alkaline to denature the DNA, neutralized, bound nitrocellulose filters 3-mm diameter “spots” a special 96-well filtration apparatus. detected by its ability hybridize 32P-labeled prepared from recombinant plasmids containing complete genome. After hybridization, washed autoradiographed; produce spots X-ray film whose intensity (in linear exposure range film) proportional amount sample. specific sensitive, correlates infectivity sera titered chimpanzees as well biophysical parameters, agreement serological indicators presence.
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