Solid-phase synthesis of polynucleotides

摘要: A commercially available resin such as polystyrene is converted into a phthalimidomethyl-resin by treatment with potassium phthalimide. The an amino hydrazine in ethanol. then combined activated ester of nucleoside to obtain amide-bonded dimethoxytrityl resin. the obtained reacting succinic anhydride presence 4-(dimethylamino) pyridine provide monosuccinate derivative, which turn treated pentachlorophenol and dicyclohexylcarbodimide dimethylformamide. Any unreacted groups may be masked group removed solution benzenesulfonic acid hydroxylated support. Additional mononucleotides, dinucleotides trinucleotides added support coupling reagent 2,4,6-triisopropylbenzenesulfonyl tetrazolide. 5'-hydroxyl acetic anhydride. steps described previous two sentences repeated form polynucleotides any desired sequence. also polyacrylmorpholide. This ethylene diamine glycol. silica gel. gel above methods, solid-phase for nucleotides.