Electron transport controls transcription of the thioredoxin gene (trxA) in the cyanobacterium Synechocystis sp. PCC 6803.

摘要: The trxA gene encoding one of the different thioredoxins facultative heterotrophic cyanobacterium Synechocystis sp. PCC 6803 is transcribed as a single mRNA 450 nucleotides. Transcript accumulation similar in all standard growth conditions but strongly decreases after transferring cell cultures from light to darkness. In steady-state conditions, transcription reduced at high (150–500 μE m−2 s−1) compared with moderate (10–50 intensities. stability transcript was intensities, and also Photosynthetic electron transport inhibitors, well glucose starvation mutant strain lacking photosystem II, promote strong decline level transcript. Primer extension analysis suggests that two proximal promoters containing −10 TATA box Escherichia coli consensus promoters. Unlike mRNA, amount thioredoxin protein not dark, neither indicating very stable. Our results indicate encoded by likely be primarily regulated transcriptional level, rather than generated photosynthetically or metabolism.