A novel method for sensitive and specific detection of DNA methylation biomarkers based on DNA restriction during PCR cycling.

摘要: DNA methylation is an important epigenetic mechanism involved in fundamental biological processes such as development, imprinting, and carcino-genesis. For these reasons, represents a valuable source for cancer biomarkers. Methods the sensitive specific detection of methylated are prerequisite implementation biomarkers into clinical routine when early based on analysis body fluids desired. Here, novel technique presented biomarkers, real-time PCR bisulfite-treated template with enzymatic digestion background during amplification using heat-stable enzyme Tsp509I. An assay lung biomarker BARHL2 was used to show analytical performance method comparison methylation-specific technology. Both technologies showed comparable analyzing technical mixtures bronchial lavage samples from 75 patients suspected having cancer. The results demonstrate that approach useful few copies high unmethylated DNA, fluids.