Drosophila PAF1 Modulates PIWI/piRNA Silencing Capacity
作者： Josef P. Clark , Reazur Rahman , Nachen Yang , Linda H. Yang , Nelson C. Lau
DOI: 10.1016/J.CUB.2017.07.052
关键词:
摘要: Summary To test the directness of factors in initiating PIWI-directed gene silencing, we employed a Piwi-interacting RNA (piRNA)-targeted reporter assay Drosophila ovary somatic sheet (OSS) cells [1]. This confirmed direct silencing roles for piRNA biogenesis and PIWI-associated [2–12] but suggested that chromatin-modifying proteins may act downstream initial event. Our data also revealed RNA-polymerase-II-associated like PAF1 RTF1 antagonize silencing. knockdown enhances PIWI reporters when piRNAs target transcript region proximal to promoter. Loss suppresses endogenous transposable element (TE) maturation, whereas subset transcripts long-non-coding RNAs adjacent TE insertions are affected by similar fashion piRNA-targeted reporters. Additionally, transcription activation at specific TEs TE-adjacent loci during is suppressed levels both reduced. study suggests a mechanistic conservation between fission yeast repressing AGO1/small interfering (siRNA)-directed [13, 14] opposing PIWI/piRNA-directed
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