Isolation, characterization of bacteriophages specific to Microlunatus phosphovorus and their application for rapid host detection
作者： S.H. Lee , M. Onuki , H. Satoh , T. Mino
DOI: 10.1111/J.1472-765X.2006.01840.X
关键词:
摘要: Aims: To isolate and characterize lytic-bacteriophages specific to Microlunatus phosphovorus, prepare fluorescently labelled phages (FLPs) for the rapid detection of host bacterium in activated sludge. Methods Results: Isolation bacteriophages lytic M. phosphovorus was attempted by applying supernatants sludge processes on lawn JCM9379 plaque formation. Thirteen bacteriophage isolates were obtained. The restriction fragment length polymorphism analysis distinguished them into two different designated as φMP1 φMP2. They found possess double-stranded DNA specificity. Morphological observations done electron microscopy. particles stained SYBR Green I shown be applicable detect their bacterial cells mixed with sludge. Conclusions: Two phosphovorus-specific isolated classified Siphoviridae. FLPs prepared, successfully applied added sludge. Significance Impact Study: At least some bacteria are susceptible related bacteriophages. Bacteriophages could affecting population sludge. used easy-rapid
unirioja.es
sci-hub.se 参考文章(16)
Donald L. Ewert, M. J. B. Paynter, Enumeration of Bacteriophages and Host Bacteria in Sewage and the Activated-Sludge Treatment Process Applied and Environmental Microbiology. ,vol. 39, pp. 576- 583 ,(1980) , 10.1128/AEM.39.3.576-583.1980
T. Mino, M.C.M. van Loosdrecht, J.J. Heijnen, Microbiology and biochemistry of the enhanced biological phosphate removal process Water Research. ,vol. 32, pp. 3193- 3207 ,(1998) , 10.1016/S0043-1354(98)00129-8
Kazunori Nakamura, Sinsuke Ishikawa, Mamoru Kawaharasaki, Phosphate uptake and release activity in immobilized polyphosphate-accumulating bacterium Microlunatus phosphovorus strain NM-1 Journal of Fermentation and Bioengineering. ,vol. 80, pp. 377- 382 ,(1995) , 10.1016/0922-338X(95)94207-8
P. A. Mosier-Boss, S. H. Lieberman, J. M. Andrews, F. L. Rohwer, L. E. Wegley, M. Breitbart, Use of fluorescently labeled phage in the detection and identification of bacterial species. Applied Spectroscopy. ,vol. 57, pp. 1138- 1144 ,(2003) , 10.1366/00037020360696008
K. NAKAMURA, A. HIRAISHI, Y. YOSHIMI, M. KAWAHARASAKI, K. MASUDA, Y. KAMAGATA, Microlunatus phosphovorus gen. nov., sp. nov., a new gram-positive polyphosphate-accumulating bacterium isolated from activated sludge. International Journal of Systematic and Evolutionary Microbiology. ,vol. 45, pp. 17- 22 ,(1995) , 10.1099/00207713-45-1-17
N. Cresswell, P. R. Herron, V. A. Saunders, E. M. H. Wellington, The fate of introduced streptomycetes, plasmid and phage populations in a dynamic soil system. Microbiology. ,vol. 138, pp. 659- 666 ,(1992) , 10.1099/00221287-138-4-659
Robert J. Seviour, Takashi Mino, Motoharu Onuki, The microbiology of biological phosphorus removal in activated sludge systems. Fems Microbiology Reviews. ,vol. 27, pp. 99- 127 ,(2003) , 10.1016/S0168-6445(03)00021-4
Jed A. Fuhrman, Marine viruses and their biogeochemical and ecological effects Nature. ,vol. 399, pp. 541- 548 ,(1999) , 10.1038/21119
Kevin E Ashelford, Martin J Day, Mark J Bailey, Andrew K Lilley, John C Fry, In Situ Population Dynamics of Bacterial Viruses in a Terrestrial Environment Applied and Environmental Microbiology. ,vol. 65, pp. 169- 174 ,(1999) , 10.1128/AEM.65.1.169-174.1999
K P Hennes, C A Suttle, A M Chan, Fluorescently Labeled Virus Probes Show that Natural Virus Populations Can Control the Structure of Marine Microbial Communities Applied and Environmental Microbiology. ,vol. 61, pp. 3623- 3627 ,(1995) , 10.1128/AEM.61.10.3623-3627.1995