[56] Uridine phosphorylase from rat liver

摘要: Publisher Summary This chapter describes the purification procedure of uridine phosphorylase enzyme from rat liver. Uridine occupies an important amphibolic position in metabolism because its role degradation pyrimidine nucleosides as well salvage pathway for nucleic acid synthesis. Separation this thymidine was achieved first Escherichia coli and later mammalian tissues. The resides mainly cytosol fraction Other assay methods arsenolysis detection uracil or ribose formation suffer disadvantages that activity is nonlinear with time, precision low. Nuclei unbroken cells are sedimented at 900 g 20 min a Sorvall RC-2B centrifuge. cytoplasmic removed; sediment rehomogenized two times weight sucrose solution recentrifuged. Thymidine phosphorylase, on other hand, has additional transferase designated direct.