作者: Felix B. Fritschi , M. Andrew Walker , Hong Lin
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摘要: The xylem-limited bacterium Xylella fastidiosa is the causal agent of Pierce's disease (PD) in grapevines, for which breeding resistant cultivars will be a long-term management strategy that involves identification and characterization germplasm. A genetically diverse group grape species selections was mechanically inoculated with X. fastidiosa, grown greenhouse 113 days after inoculation, evaluated levels bacterial concentrations stem leaf tissues by quantitative enzyme-linked immunosorbent assay (ELISA). Concentrations were affected genotype, plant tissue, position on relative to point interactions among these factors. Based estimated samples at d postinoculation, 9621-67, Muscadinia rotundifolia, Vitis arizonica/candicans, V. arizonica/girdiana, candicans, girdiana, nesbittiana, shuttleworthii PD. In contrast, vinifera, aestivalis, 9621-94, champinii had very high tissues. Sequential sampling blades 34, 77, postinoculation revealed different temporal patterns genotypes. Estimates decreased first M. arizonica/candicans but increased all other broad range genotypes allows selection promising genetic backgrounds capable greatly limiting population size development stems, critical trait PD-resistant grapevines.