Staphylococcus aureus Enterotoxin B Release (Excretion) Under Controlled Conditions of Fermentation

摘要: Release of Staphylococcus aureus enterotoxin B (SEB) into the culture medium was initiated during mid-log phase growth. A consisting 4% N-Z Amine (Sheffield), 0.2% dextrose, and 1% yeast extract supported maximum production SEB. Although pH cultivation did not significantly affect growth curve organism, time required for detectable excretion affected, as final yield. Optimal conditions SEB were achieved with control at 7.0; alkaline (pH 8.0) produced only minimal amounts toxin, whereas acid 6.0) resulted in 50% reduction Slightly less when there no extrinsic control, cultures buffered by media constituents by-products With 7.0, deletion dextrose from 40% 8-h There also a delay early stages fermentation.