Highly sensitive method for detecting mutated gene
作者： Akira Ohide , Hideo Matsumoto , Hideya Fujimoto , Koichiro Matsuda
DOI:
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摘要: Various highly sensitive detection methods, particularly improved PNA-LNA-PCR clamp are provided as methods for detecting the presence or absence of a mutated gene contained in pool rapidly, simple manner, with high accuracy, and sensitivity. As step before main detection, pre-amplification comprising allowing (1) primer consisting PNA which hybridizes all part target site having sequence wild-type complementary to gene, (2) capable amplifying region (3) coexist reaction solution amplification, selectively by amplification method.
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