摘要: Publisher Summary This chapter describes reflection interference contrast microscopy (RICM). The term “RICM” is used in the to cover all optical configurations and practical applications of method. method by which light reflected from a semitransparent, epi-illuminated specimen contributes image formation microscope has been given different names, depending on details configuration particular application. It was introduced into cell biology Curtis using name “interference microscopy” (IRM), this name, or its variant, “reflection (RIM), often studies adhesion living cells. Ploem (RCM) introduction antiflex increase contrast. In RICM cells, reflections plasma membrane intracellular membranes contribute interfering with each other interface between underlying glass surface liquid medium.
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