Cloning and expression of neuron-specific enolase in the corpus luteum of dairy goats.
作者： Xia Meng , Feihu Li , Shulin Chen , Caiyan Tang , Wenhua Zhang
DOI: 10.1016/J.GENE.2012.04.058
关键词:
摘要: Neuron-specific enolase (NSE) is the key molecular marker for diffuse neuroendocrine system (DNES) cells, its expression in pregnant corpus luteum (CL) of dairy goats was studied by immunofluorescence method and ultra structural characteristics luteal cells were detected electron microscopy to identify existence DNES CL goats. Besides, coding sequence NSE gene cloned biological information analyzed. Results revealed that immunopositive exhibited widespread cytoplasmic staining throughout whole CL. In addition, these showed typical microscopy. These results suggested many exist Meanwhile, we identified (GenBank Accession No. JN887466). Its nucleotide homology found be 97.9%, 89.3%, 90% 92.6%, respectively, compared with Bos taurus, Rattus norvegicus, Mus musculus Homo sapiens, while amino acid 99.1%, 97%, 97.2% 98.2% respectively. first functional acids coded highly conserved Caprine, B. R. M. H. sapiens. It implied had close other species. Our findings demonstrated possible CL, providing new clue further understanding interactions between reproductive systems. Characterization will enable us synthesize interference RNA study on role formation, function apoptosis
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sci-hub.se 参考文章(26)
J M Polak, S R Bloom, Regulatory peptides of the gastrointestinal and respiratory tracts. Archives internationales de pharmacodynamie et de thérapie. ,vol. 280, pp. 16- 49 ,(1986)
R. Ivell, D. Richter, The gene for the hypothalamic peptide hormone oxytocin is highly expressed in the bovine corpus luteum: biosynthesis, structure and sequence analysis. The EMBO Journal. ,vol. 3, pp. 2351- 2354 ,(1984) , 10.1002/J.1460-2075.1984.TB02139.X
Geqing Chai, John M. Brewer, Leslie L. Lovelace, Takashi Aoki, Wladek Minor, Lukasz Lebioda, Expression, Purification and the 1.8Å Resolution Crystal Structure of Human Neuron Specific Enolase Journal of Molecular Biology. ,vol. 341, pp. 1015- 1021 ,(2004) , 10.1016/J.JMB.2004.05.068
Michael R Tracy, S.Blair Hedges, Evolutionary history of the enolase gene family. Gene. ,vol. 259, pp. 129- 138 ,(2000) , 10.1016/S0378-1119(00)00439-X
Ryosuke Sakumoto, Tokushi Komatsu, Etsuko Kasuya, Toshiyuki Saito, Kiyoshi Okuda, Expression of mRNAs for interleukin-4, interleukin-6 and their receptors in porcine corpus luteum during the estrous cycle Domestic Animal Endocrinology. ,vol. 31, pp. 246- 257 ,(2006) , 10.1016/J.DOMANIEND.2005.11.001
KENNETH P. McNATTY, ANASTASIA MAKRIS, CAMILLO DEGRAZIA, OSATHANONDH RAPIN, KENNETH J. RYAN, The production of progesterone, androgens, and estrogens by granulosa cells, thecal tissue, and stromal tissue from human ovaries in vitro. The Journal of Clinical Endocrinology and Metabolism. ,vol. 49, pp. 687- 699 ,(1979) , 10.1210/JCEM-49-5-687
Levent M Senturk, Emre Seli, Linda S Gutierrez, Gil Mor, Hulusi B Zeyneloglu, Aydin Arici, Monocyte chemotactic protein-1 expression in human corpus luteum. Molecular Human Reproduction. ,vol. 5, pp. 697- 702 ,(1999) , 10.1093/MOLEHR/5.8.697
Lukasz Lebioda, Boguslaw Stec, Crystal structure of enolase indicates that enolase and pyruvate kinase evolved from a common ancestor. Nature. ,vol. 333, pp. 683- 686 ,(1988) , 10.1038/333683A0
Sybil M. McALEESE, Bryan DUNBAR, John E. FOTHERGILL, Lesley J. HINKS, Ian N.M. DAY, Complete amino acid sequence of the neurone-specific gamma isozyme of enolase (NSE) from human brain and comparison with the non-neuronal alpha form (NNE). FEBS Journal. ,vol. 178, pp. 413- 417 ,(1988) , 10.1111/J.1432-1033.1988.TB14465.X
V. Pancholi, Multifunctional α-enolase: its role in diseases Cellular and Molecular Life Sciences. ,vol. 58, pp. 902- 920 ,(2001) , 10.1007/PL00000910