Nuclear envelope fraction proteins: Isolation and comparison with the nuclear protein of the avian erythrocyte

作者: Keith R. Shelton , Carrington S. Cobbs , John T. Povlishock , Rosalind K. Burkat

DOI: 10.1016/0003-9861(76)90336-2

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摘要: Abstract A nuclear envelope fraction is obtained from circulating erythrocytes of mature chickens. This was characterized by chemical analysis, ultramicroscopic examination and electrophoretic analysis the polypeptides. It free plasma membrane polypeptides, hemoglobin deoxyribonucleohistone. Comparisons sodium dodecyl sulfate-polyacrylamide-gel electropherograms polypeptides with either chromosomal or total nonhistone Triton X-100-washed nuclei indicate that certain major are common to all fractions. indicates many chicken erythrocyte “chromosomal” may be primarily associated structural elements which not, in fact, Although this finding cannot extended per se other cells, it does suggest caution should exercised describing components as when designation intended imply primary association further study will provide new insights into structure function.

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