High-throughput and quantitative genome-wide messenger RNA sequencing for molecular phenotyping.
作者： John E Collins , Neha Wali , Ian M Sealy , James A Morris , Richard J White
DOI: 10.1186/S12864-015-1788-6
关键词:
摘要: We present a genome-wide messenger RNA (mRNA) sequencing technique that converts small amounts of from many samples into molecular phenotypes. It encompasses all steps sample preparation to sequence analysis and is applicable baseline profiling or perturbation measurements. Multiplex transcript 3′ ends identifies differential abundance independent gene annotation. show increasing biological replicate number while maintaining the total amount more differentially abundant transcripts. This method can be implemented on polyadenylated any organism with an annotated reference genome in laboratory access Illumina sequencing.
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