Effects of naringin on the proliferation and osteogenic differentiation of human amniotic fluid‐derived stem cells
作者： Meimei Liu , Yan Li , Shang-Tian Yang
DOI: 10.1002/TERM.1911
关键词:
摘要: Human amniotic fluid-derived stem cells (hAFSCs) are a novel cell source for generating osteogenic to treat bone diseases. Effective induction of differentiation from hAFSCs is critical fulfil their therapeutic potential. In this study, naringin, the main active compound Rhizoma drynariae (a Chinese herbal medicine), was used stimulate proliferation and hAFSCs. The results showed that naringin enhanced alkaline phosphatase activity (ALP) in dose-dependent manner range 1-100 µg/ml, while an inhibition effect observed at 200 µg/ml. Consistently, calcium content also increased with concentration up 100 by further confirmed upregulation marker genes, including osteopontin (OPN) Collagen I RT-PCR analysis. osteoprotegerin (OPG) expression minimal receptor activator nuclear factor-κB ligand (RANKL) suggested inhibited osteoclastogenesis addition, gene expressions morphogenetic protein 4 (BMP4), runt-related transcription factor 2 (RUNX2), β-catenin Cyclin D1 significantly, indicating promotes osteogenesis via BMP Wnt-β-catenin signalling pathways. These can be upregulate hAFSCs, which could provide attractive promising treatment disorders. Copyright © 2014 John Wiley & Sons, Ltd.
sci-hub.se 参考文章(50)
Xiaorong Ma, Shengli Zhang, Junmei Zhou, Baisong Chen, Yafeng Shang, Tongbing Gao, Xue Wang, Hua Xie, Fang Chen, None, Clone-derived human AF-amniotic fluid stem cells are capable of skeletal myogenic differentiation in vitro and in vivo Journal of Tissue Engineering and Regenerative Medicine. ,vol. 6, pp. 598- 613 ,(2012) , 10.1002/TERM.462
Seth M Arum, New developments surrounding the safety of bisphosphonates. Current Opinion in Endocrinology, Diabetes and Obesity. ,vol. 15, pp. 508- 513 ,(2008) , 10.1097/MED.0B013E3283184017
Lucinda Strycker Orsini, Matthew D. Rousculp, Stacey R. Long, Shaohung Wang, Health care utilization and expenditures in the United States: a study of osteoporosis-related fractures Osteoporosis International. ,vol. 16, pp. 359- 371 ,(2005) , 10.1007/S00198-004-1694-2
Meimei Liu, Yan Li, Shang-Tian Yang, Expansion of human amniotic fluid stem cells in 3-dimensional fibrous scaffolds in a stirred bioreactor Biochemical Engineering Journal. ,vol. 82, pp. 71- 80 ,(2014) , 10.1016/J.BEJ.2013.11.001
Marcus Egermann, J. Goldhahn, E. Schneider, Animal models for fracture treatment in osteoporosis. Osteoporosis International. ,vol. 16, pp. 129- 138 ,(2005) , 10.1007/S00198-005-1859-7
Márcia T. Rodrigues, Sang Jin Lee, Manuela E. Gomes, Rui L. Reis, Anthony Atala, James J. Yoo, Amniotic fluid-derived stem cells as a cell source for bone tissue engineering. Tissue Engineering Part A. ,vol. 18, pp. 2518- 2527 ,(2012) , 10.1089/TEN.TEA.2011.0672
Yi-Chun Yeh, Hao-Ji Wei, Wen-Yu Lee, Chu-Leng Yu, Yen Chang, Li-Wen Hsu, Min-Fan Chung, Ming-Song Tsai, Shiaw-Min Hwang, Hsing-Wen Sung, Cellular Cardiomyoplasty with Human Amniotic Fluid Stem Cells: In Vitro and In Vivo Studies Tissue Engineering Part A. ,vol. 16, pp. 1925- 1936 ,(2010) , 10.1089/TEN.TEA.2009.0728
Brendan F. Boyce, Lianping Xing, FUNCTIONS OF RANKL/RANK/OPG IN BONE MODELING AND REMODELING Archives of Biochemistry and Biophysics. ,vol. 473, pp. 139- 146 ,(2008) , 10.1016/J.ABB.2008.03.018
Timothy F. Day, Xizhi Guo, Lisa Garrett-Beal, Yingzi Yang, Wnt/β-Catenin Signaling in Mesenchymal Progenitors Controls Osteoblast and Chondrocyte Differentiation during Vertebrate Skeletogenesis Developmental Cell. ,vol. 8, pp. 739- 750 ,(2005) , 10.1016/J.DEVCEL.2005.03.016
Smitha Mathews, Ramesh Bhonde, Pawan Kumar Gupta, Satish Totey, Extracellular matrix protein mediated regulation of the osteoblast differentiation of bone marrow derived human mesenchymal stem cells. Differentiation. ,vol. 84, pp. 185- 192 ,(2012) , 10.1016/J.DIFF.2012.05.001