Down-regulation of expression and function of nucleoside diphosphate kinase in insulin-secreting β-cells under in vitro conditions of glucolipotoxicity

摘要: Previously, we reported a significant reduction in expression and the activity of nucleoside diphosphate kinase (NDP kinase) islets derived from Goto-Kakizaki rat (GK rat), an animal model for type 2 diabetes. Herein, examined effects chronic exposure insulin-secreting β-(INS 832/13) cells to high glucose (a glucotoxicity), palmitate lipotoxicity), or plus glucolipotoxicity) on nm23-H1 A) nm23-H2 B). Our findings indicate marked both associated NDP under each these conditions. A cell-permeable analog ceramide (CER) also mimicked significantly reducing cells. These suggest that de novo generation intracellular CER might represent at least one signaling steps involved lipid-induced function β-cells. Based data, conclude glucolipotoxic conditions impair pancreatic Potential significance findings, specifically level abnormal G-protein activation impaired insulin secretion is discussed.