Differential Proliferative and Interleukin-10 Responses to Fractionated Filarial Antigens: Preferential Recognition by Patients with Chronic Lymphatic Dysfunction
作者： K. A. Dimock , D. G. Addiss , M. L. Eberhard , P. J. Lammie
关键词:
摘要: To characterize filarial antigens that may be associated with the development of chronic lymphatic dysfunction in persons filariasis, T cell responsiveness to Brugia pahangi adult worm extracts and SDS-PAGE antigen fractions were examined among Haitians from an area which Wuchereria bancrofti is endemic. Greater proliferation interleukin-10 (IL-10) production observed amicrofilaremic patients hydrocele or elephantiasis than microfilaremic asymptomatic persons. Antigen stimulated highest proliferative responses (in 25-49 kDa range) IL-10 not identical. Further separation immunodominant 30- 38-kDa fraction by ion exchange high-pressure liquid chromatography identified several subfractions, including a 32-kDa protein band, elicited hydrocele. By immunoblot, these also had markedly greater humoral reactivity parasite approximately 52, 43, 32, 30 kDa.
oup.com
oxfordjournals.org
sci-hub.se 参考文章(20)
Phillip Scott, Edward Pearce, Allen W. Cheever, Robert L. Coffman, Alan Sher, Role of cytokines and CD4+ T-cell subsets in the regulation of parasite immunity and disease Immunological Reviews. ,vol. 112, pp. 161- 182 ,(1989) , 10.1111/J.1600-065X.1989.TB00557.X
E.A. Ottesen, Immunological aspects of lymphatic filariasis and onchocerciasis in man. Transactions of The Royal Society of Tropical Medicine and Hygiene. ,vol. 78, pp. 9- 18 ,(1984) , 10.1016/0035-9203(84)90309-2
Edith Cookson, Liang Tang, Murray E. Selkirk, Conservation of primary sequence of gp29, the major soluble cuticular glycoprotein, in three species of lymphatic filariae Molecular and Biochemical Parasitology. ,vol. 58, pp. 155- 159 ,(1993) , 10.1016/0166-6851(93)90099-J
P. J. Lammie, D. G. Addiss, G. Leonard, A. W. Hightower, M. L. Eberhard, Heterogeneity in Filarial-Specific Immune Responsiveness among Patients with Lymphatic Obstruction The Journal of Infectious Diseases. ,vol. 167, pp. 1178- 1183 ,(1993) , 10.1093/INFDIS/167.5.1178
U. K. LAEMMLI, Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Nature. ,vol. 227, pp. 680- 685 ,(1970) , 10.1038/227680A0
T B Nutman, T J Lynch, R B Lal, Brugia malayi antigens associated with lymphocyte activation in filariasis. Journal of Immunology. ,vol. 139, pp. 1652- 1657 ,(1987)
MM Bradford, None, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Analytical Biochemistry. ,vol. 72, pp. 248- 254 ,(1976) , 10.1016/0003-2697(76)90527-3
E. Cookson, M. L. Blaxter, M. E. Selkirk, Identification of the major soluble cuticular glycoprotein of lymphatic filarial nematode parasites (gp29) as a secretory homolog of glutathione peroxidase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 89, pp. 5837- 5841 ,(1992) , 10.1073/PNAS.89.13.5837
T R Mosmann, R L Coffman, TH1 and TH2 Cells: Different Patterns of Lymphokine Secretion Lead to Different Functional Properties Annual Review of Immunology. ,vol. 7, pp. 145- 173 ,(1989) , 10.1146/ANNUREV.IY.07.040189.001045
Albert L. Vincent, Ann C. Vickery, Michael J. Lotz, Usha Desai, The lymphatic pathology of Brugia pahangi in nude (athymic) and thymic mice C3H/HeN. Journal of Parasitology. ,vol. 70, pp. 48- 56 ,(1984) , 10.2307/3281924