Lysyl Oxidase Gene Expression and Enzyme Activity in the Rat Ovary: Regulation by Follicle-Stimulating Hormone, Androgen, and Transforming Growth Factor-β Superfamily Members in Vitro

摘要: Lysyl oxidase (LOX) catalyzes the final enzymatic reaction required for cross-linking of collagen and elastin fibers therefore has a crucial role in regulating formation maintenance extracellular matrix ovary. LOX mRNA is abundantly expressed rat granulosa cells. To examine how regulation ovary might influence follicular development, we studied expression enzyme activity cells from late preantral/early antral follicles vitro. FSH dose dependently inhibited (50% reduction at 10 ng/ml) vitro, action was mimicked by 8-bromo-cAMP, suggesting via elevation cAMP. Dihydrotestosterone alone enhanced activity, but potentiated effect FSH, causing further reduction. TGFbeta1 (5-fold increase (1.5-fold increase). caused (by up to 84% 80%, respectively). Growth differentiation factor-9 (GDF-9) activin A, same concentration as (10 ng/ml), stimulated within 6 h, although overall higher 48 h. All three factors when combined with reduced both 60%) compared alone. These findings indicate control endocrine, paracrine, autocrine levels suggest coordinated ovarian during determining whether local act stimulators or inhibitors LOX.