An In Vivo 11C-(R)-PK11195 PET and In Vitro Pathology Study of Microglia Activation in Creutzfeldt-Jakob Disease

摘要: Microgliosis is part of the immunobiology Creutzfeldt-Jakob disease (CJD). This first report using 11C-(R)-PK11195 PET imaging in vivo to measure 18 kDa translocator protein (TSPO) expression, indexing microglia activation, symptomatic CJD patients, followed by a postmortem neuropathology comparison. One genetic (gCJD) patient, two sporadic (sCJD) one variant (vCJD) patient (mean ± SD age, 47.50 ± 15.95 years), and nine healthy controls 44.00 ± 11.10 years) were included study. TSPO binding potentials estimated clustering parametric analyses reference regions. Statistical comparisons run at regional voxel-wise levels. Postmortem evaluation measured scrapie prion (PrPSc) immunoreactivity, neuronal loss, spongiosis, astrogliosis, microgliosis. 11C-(R)-PK11195-PET showed significant overexpression cortical level sCJD as well thalamic cerebellar involvement; very limited parieto-occipital activation gCJD case; increases subcortical thalamus, basal ganglia, midbrain cerebellum vCJD brain. Along with misfolded deposits, all patients revealed spongiosis diffuse cerebral microgliosis which was particularly dense ganglia structures These findings confirm CJD, variably modulated more evaluation. Thus, topography, extent can vary subtypes, shown vivo, possibly related response fast apoptotic processes, but reaches large amount final course.