Detection of the carbapenemase gene bla VIM-5 in members of the Pseudomonas putida group isolated from polluted Nigerian wetlands

摘要: There are increasing concerns about possible dissemination of clinically relevant antibiotic resistance genes, including genes encoding for carbapenemases in the environment. However, little is known environmental distribution Africa. In this study, four polluted urban wetlands Nigeria were investigated as potential reservoirs carbapenem-resistant bacteria (CRB). CRB isolated from wetlands, characterized by Blue-Carba test, MIC determinations and whole genome sequencing (WGS). Nine 65 bacterial isolates identified members Pseudomonas putida group (P. plecoglossicida P. guariconensis, respectively) harboured metallo-beta-lactamase gene blaVIM-5. WGS revealed blaVIM-5 three novel Tn402-like class 1 integron structures containing cassette arrays aadB|blaVIM-5|blaPSE-1, aadB|blaVIM-5|aadB|blaPSE-1, blaVIM-5|aadB|tnpA|blaPSE-1|smr2|tnpA, respectively. Strains carrying aadB|blaVIM-5|blaPSE-1 also carried an identical without addition, strains another bcr2, several multidrug efflux pumps, at least one ampC, aph(3")-lb, aph(6)-ld, tetB, tetC, tetG, floR, macAB. This first report a carbapenemase sources isolates. result underscores role Nigerian environment reservoir genes.