Protein biomarker analysis of primary Peyronie's disease cells

摘要: Introduction. The molecular pathogenesis of Peyronie's Disease (PD) remains unclear more than 250 years after its initial description. Because this, no test is currently available to accurately predict PD progression among those affected. Aim. To investigate the expression wound healing and fibrosis-associated proteins in primary cell cultures fibroblasts determine whether altered protein patterns can be used as predictors clinical course natural history. Methods. Primary derived from normal Tunica albuginea tissue plaque were examined by immuno-cytochemistry. Protein profiles analyzed Surface-Enhanced Laser Desorption/ Ionization Time-of-Flight Mass Spectrometry (SELDI-TOF-MS) Western immunoblotting. Main Outcome Measures. Expression assessed. Results. Statistically significant increases smooth muscle a-actin, b-catenin, Heat shock (Hsp47) identified cells relative tissue. Changes TGFb-1 receptor Fibronectin also observed. In addition, additional yet unidentified at 4.7, 8.9, 10.8, 16.8, 76.8 kDa detected complementary SELDI-TOF-MS approaches. Conclusions. plaques display up-regulated several that are established components fibrosis healing. changes other, measured. It will interest conduct further studies see these dysregulated peaks represent potential biological markers disease progression. De Young LX, Bella AJ, O'Gorman DB, Gan BS, Lim KB, BrockGB. biomarker analysis cells. J Sex Med 2010;7:99-106.