PcFKH1, a novel regulatory factor from the forkhead family, controls the biosynthesis of penicillin in Penicillium chrysogenum.

摘要: Penicillin biosynthesis in Penicillium chrysogenum (re-identified as rubens) is a good example of biological process subjected to complex global regulatory networks and serves model study fungal secondary metabolism. The winged-helix family transcription factors recently described, which includes the forkhead type proteins, key proteins involved this process. In yeasts humans, are different processes (cell cycle regulation, cell death control, pre-mRNA processing morphogenesis); one member has been identified P. genome (Pc18g00430). work, we have characterized novel factor (named PcFKH1) by generating knock-down mutants overexpression strains. Results clearly indicate that PcFKH1 positively controls antibiotic through specific interaction with promoter region penDE gene, thus regulating mRNA levels. also binds pcbC promoter, but low affinity. addition, it other ancillary genes penicillin biosynthetic process, such phlA (encoding phenylacetyl CoA ligase) ppt phosphopantetheinyl transferase). plays role conidiation spore pigmentation, does not seem be hyphal morphology or division improved laboratory reference strain Wisconsin 54-1255. A genome-wide analysis putatively coregulated PcRFX1 (another factor) provided evidence effect these