Comparison of microRNA Expression Profile in Chronic Myeloid Leukemia Patients Newly Diagnosed and Treated by Allogeneic Hematopoietic Stem Cell Transplantation.

摘要: Chronic myeloid leukemia (CML) results from a translocation between chromosomes 9 and 22, which generates the Philadelphia chromosome. This forms BCR/ABL1, an active tyrosine kinase protein that promotes cell growth replication. Despite great progress in CML treatment form of inhibitors, allogeneic-hematopoietic stem transplantation (allo-HSCT) is currently used as important alternative for patients resistant to these inhibitors. Studies have shown unregulated expression microRNAs, act oncogenes or tumor suppressors, associated with human cancers. contributes formation development by stimulating proliferation, angiogenesis, invasion. Research has demonstrated potential microRNAs biomarkers cancer diagnosis, prognosis, therapeutic targets. In present study, we compared circulating microRNA profiles 14 newly diagnosed chronic phase-CML chromosome-negative after allo-HSCT. For each patient, tested 758 reverse transcription quantitative polymerase chain reaction (RT-qPCR) analysis. The global profile revealed 16 upregulated 30 downregulated microRNAs. Target genes were analyzed, key pathways extracted compared. Bioinformatics tools analyze data. Among miRNA target genes, some related proliferation identified. These reveal comprehensive main miRNAs cytogenetic remission provide new resources exploring transplantation-based strategies.