Dynamic Monte Carlo simulations to model FRET and photobleaching in systems with multiple donor-acceptor interactions
作者： Patrick L. T. M. Frederix , Evert L. de Beer , Wendie Hamelink , Hans C. Gerritsen
DOI: 10.1021/JP014549B
关键词:
摘要: Monte Carlo simulations were employed to model FRET in systems that included interactions between multiple donors and acceptors photobleaching. Simulations revealed pixel-to-pixel variations microscopy experiments can be due both the probe distribution photon noise, depending on intensity probes per detection pixel. The used describe fluorescence (single) actin filaments using a conventional microscope. labeled with TRITC at phalloidin Cy5-maleimide cys374 donor−acceptor distances taken from literature. images show large variation suffer In experiments, main source of noise was distribution. acceptor suffered rapid photobleaching, which resulted an increase donor time hampers straightforward determination energy transfer efficienc...
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