Detection of alkaline phosphatase in canine cells previously stained with Wright-Giemsa and its utility in differentiating osteosarcoma from other mesenchymal tumors.
作者： Julia K. Ryseff , Andrea A. Bohn
DOI: 10.1111/J.1939-165X.2012.00445.X
关键词:
摘要: Background Osteosarcoma (OSA) is a common primary bone tumor in dogs. Demonstration of alkaline phosphatase (ALP) reactivity by cells on unstained slides useful differentiating osteosarcoma from other types sarcoma. However, are not always available. Objectives The objectives this study were to evaluate the diagnostic utility detecting ALP expression sarcomas dogs using cytologic material previously stained with Wright–Giemsa stain and assess sensitivity specificity for diagnosing specific protocol. Methods Archived aspirates histologically confirmed that had been treated 5-bromo, 4-chloro, 3-indolyl phosphate/nitroblue tetrazolium (BCIP/NBT) as substrate ALP. Cells evaluated after incubation BCIP/NBT 1 hour. Sensitivity diagnosis OSA calculated. Results In samples 83 dogs, 15/17 OSAs 4/66 tumors than (amelanotic melanoma, gastrointestinal stromal tumor, collision anaplastic sarcoma) expressed detected applied 88 94%, respectively. Conclusions ALP canine mesenchymal neoplasms.
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