Sequence information can be obtained from single DNA molecules

作者: I. Braslavsky , B. Hebert , E. Kartalov , S. R. Quake

DOI: 10.1073/PNAS.0230489100

关键词:

摘要: … to sequence single DNA molecules, but none have been experimentally demonstrated. Here we report the use of DNA polymerase to obtain sequence information from … incorporation of …

参考文章(33)
Emil P. Kartalov, Marc A. Unger, Stephen R. Quake, Polyelectrolyte surface interface for single-molecule fluorescence studies of DNA polymerase. BioTechniques. ,vol. 34, pp. 505- 510 ,(2003) , 10.2144/03343ST02
M. Unger, E. Kartalov, C.-S. Chiu, H.A. Lester, S.R. Quake, Single-molecule fluorescence observed with mercury lamp illumination. BioTechniques. ,vol. 27, pp. 1008- 1014 ,(1999) , 10.2144/99275RR01
R. S. Trager, Venter's Next Goal: 1000 Human Genomes Science. ,vol. 298, pp. 947- 947 ,(2002) , 10.1126/SCIENCE.298.5595.947
Stefan Howorka, Stephen Cheley, Hagan Bayley, Sequence-specific detection of individual DNA strands using engineered nanopores Nature Biotechnology. ,vol. 19, pp. 636- 639 ,(2001) , 10.1038/90236
Gijs J.L. Wuite, Steven B. Smith, Mark Young, David Keller, Carlos Bustamante, Single-molecule studies of the effect of template tension on T7 DNA polymerase activity Nature. ,vol. 404, pp. 103- 106 ,(2000) , 10.1038/35003614
Lloyd M. Smith, Jane Z. Sanders, Robert J. Kaiser, Peter Hughes, Chris Dodd, Charles R. Connell, Cheryl Heiner, Stephen B. H. Kent, Leroy E. Hood, Fluorescence detection in automated DNA sequence analysis Nature. ,vol. 321, pp. 674- 679 ,(1986) , 10.1038/321674A0
A. Meller, L. Nivon, E. Brandin, J. Golovchenko, D. Branton, Rapid nanopore discrimination between single polynucleotide molecules. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 97, pp. 1079- 1084 ,(2000) , 10.1073/PNAS.97.3.1079
Michelle D Wang, Mark J Schnitzer, Hong Yin, Robert Landick, Jeff Gelles, Steven M Block, Force and Velocity Measured for Single Molecules of RNA Polymerase Science. ,vol. 282, pp. 902- 907 ,(1998) , 10.1126/SCIENCE.282.5390.902
M.A. Augustin, W. Ankenbauer, B. Angerer, Progress towards single-molecule sequencing: enzymatic synthesis of nucleotide-specifically labeled DNA. Journal of Biotechnology. ,vol. 86, pp. 289- 301 ,(2001) , 10.1016/S0168-1656(00)00420-X