Serine hydroxymethyltransferase activity and serine incorporation in leukocytes.

摘要: Studies of serine hydroxymethyltransferase activity in extracts leukocytes from normal and leukemic subjects showed that the enzyme is present lymphocytes granulocytes but higher lymphocytes. It also than patients with chronic lymphocytic leukemia to a lesser extent acute myelocytic leukemia. A striking increase occurs stimulated by phytohemagglutinin divide culture. Enzyme rises severalfold before cell number increases. Stimulated take up [3-14C]serine medium incorporate its radioactivity into DNA, RNA, other fractions. The rate incorporation increases sharply rise number. Thus, vivo show temporal correlation Inhibitors DNA synthesis ( e.g. , fluorodeoxyuridine or high concentrations adenosine thymidine) block results suggest cellular uptake have significant role proliferating cells.